Flow cytometric analysis of the effect of dithiothreitol on leukocyte surface markers
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چکیده
منابع مشابه
Flow cytometric analysis of the effect of dithiothreitol on leukocyte surface markers.
Pretreatment with dithiothreitol (DTT) is necessary to dissolve mucus in samples of induced sputum prior to analysis. However, DTT may affect cell surface markers which are essential for lymphocyte subtyping. Therefore, the aim of this study was to evaluate the effect of DTT on an appropriate panel of surface markers. Peripheral blood leukocytes were used because these cells, in contrast to spu...
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ژورنال
عنوان ژورنال: European Respiratory Journal
سال: 2000
ISSN: 0903-1936,1399-3003
DOI: 10.1034/j.1399-3003.2000.16b22.x